| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.254605 |
| Chromosome: | chromosome 1 |
| Location: | 51693 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000250 | SNR1 | (1 of 2) PTHR12677:SF5 - TVP38/TMEM64 FAMILY MEMBRANE PROTEIN YDJX; Predicted SNARE-associated Golgi protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTCCTACCGCGACAGCCTCATCAATATG |
| Internal bar code: | TTTACGGTACATGAGTTGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 330 |
| LEAP-Seq percent confirming: | 99.7626 |
| LEAP-Seq n confirming: | 2521 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTGTAACCTTGTTGGTT |
| Suggested primer 2: | GCCATGAGCTCCCATATGTT |