| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254610 |
| Chromosome: | chromosome 4 |
| Location: | 3925226 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g230928 | TRL13 | (1 of 1) K10758 - thiol oxidase (QSOX); Thioredoxin-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGAGAAGCAAGAGGGGGGTTGCAAAGAT |
| Internal bar code: | ACACAAGACGCATCAATCTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 422 |
| LEAP-Seq percent confirming: | 96.25 |
| LEAP-Seq n confirming: | 539 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGTTAGAAAACGCGAA |
| Suggested primer 2: | AATCTCACCCACACCCACAT |