| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.254612 |
| Chromosome: | chromosome 9 |
| Location: | 6436364 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407110 | (1 of 1) PTHR12461:SF16 - 2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGCCGACCCCGCTCCTCCAACTCCTCC |
| Internal bar code: | GGTTCACGATGGTAACATGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 177 |
| LEAP-Seq percent confirming: | 99.0291 |
| LEAP-Seq n confirming: | 102 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATTCGATGGGCAGAGTCC |
| Suggested primer 2: | ACCTCTCCCCCATATGGTTC |