Insertion junction: LMJ.RY0402.254613_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.254613
Chromosome:chromosome 11
Location:1154574
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGAGAAGCTGGCTCTTGATTCCGAGAAA
Internal bar code:CACGTGACGCGAGCAACCAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:487
LEAP-Seq percent confirming:98.9011
LEAP-Seq n confirming:90
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGTAGCGCACACACAAG
Suggested primer 2:CCTCATATCATGCCGTTGTG