| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254684 |
| Chromosome: | chromosome 12 |
| Location: | 1362591 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488400 | KATL5 | katanin like protein 5, similar to the catalytic subunit; (1 of 9) 3.6.4.3 - Microtubule-severing ATPase / Katanin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGATCGCTGCCGCTGTACCCTGAAACGAG |
| Internal bar code: | GAGCTGACGACAGGTGGTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 99.6364 |
| LEAP-Seq n confirming: | 274 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGAGAAGCTGTGGTAGAG |
| Suggested primer 2: | GAAAATCAGGACGAGGTGGA |