| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.254728 |
| Chromosome: | chromosome 13 |
| Location: | 1553244 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573000 | E2FR1 | (1 of 2) PTHR13271//PTHR13271:SF44 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE SMALL SUBUNIT N-METHYLTRANSFERASE I-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCCAATGCCTCACCGCGCTCCTACACG |
| Internal bar code: | GCCTCGGAACTACGCTAGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGCACACCCACATTCAC |
| Suggested primer 2: | TTTCCAAAACGTTGGAGGTC |