| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254742 |
| Chromosome: | chromosome 1 |
| Location: | 2136897 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011600 | (1 of 1) PTHR23244//PTHR24202//PTHR24202:SF4 - KELCH REPEAT DOMAIN // FAMILY NOT NAMED // E3 UBIQUITIN-PROTEIN LIGASE MIB2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAATGGAGCAATCGGACTAGATCTGCCG |
| Internal bar code: | GAATGTGTCATGTTCGCCACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 147 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGAGCATGGACGGTAGGT |
| Suggested primer 2: | CCCTGCACCTTACATACCGT |