| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254752 |
| Chromosome: | chromosome 6 |
| Location: | 1697700 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261400 | DTH1 | Delayed in Triacylglycerol Hydrolysis 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATACAGCCTGGTGCCCATCTAAAGGCACA |
| Internal bar code: | TTTGTATCCAGCGTTCTGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 179 |
| LEAP-Seq percent confirming: | 99.5017 |
| LEAP-Seq n confirming: | 1198 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTCTTTGCATGGCACGAG |
| Suggested primer 2: | GTGGCCTACATGATGACACG |