| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254757 |
| Chromosome: | chromosome_1 |
| Location: | 7969612 |
| Confidence (%): | 73 |
| Locus disrupted | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g068012 | PPR5 | Pentatrichopeptide repeat protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGAAACCCCTGCAGAGCACCTGGCATGCG |
| Internal bar code: | TCAGCCCGCCGGTGTTGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 180 |
| LEAP-Seq percent confirming: | 94.0639 |
| LEAP-Seq n confirming: | 206 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTATGCCAGTAAGGTCG |
| Suggested primer 2: | GCATAGTGGGCAAAGGTGAT |