| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254781 |
| Chromosome: | chromosome 12 |
| Location: | 1344498 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488600 | (1 of 1) IPR000104//IPR006059 - Antifreeze protein, type I // Solute-binding family 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCATACGAAACAGCGAACTGTGGGAGG |
| Internal bar code: | GCCCAAATACCTAGAATAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 220 |
| LEAP-Seq percent confirming: | 99.0385 |
| LEAP-Seq n confirming: | 103 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCCACCATAATGCTGTAA |
| Suggested primer 2: | AGTTGAGCGAGCGATTTGTT |