| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.254811 |
| Chromosome: | chromosome 9 |
| Location: | 1919236 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395200 | (1 of 1) PF04087 - Domain of unknown function (DUF389) (DUF389) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATAGCGTCCCGGCAAGGTGTCACAGGC |
| Internal bar code: | ATCGAGGACGGACTAGTCTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 88 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGAGAGCCAACTAGGGA |
| Suggested primer 2: | GGTGTGTGTGTGTGTGTGGA |