| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254839 |
| Chromosome: | chromosome 3 |
| Location: | 4663874 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177500 | (1 of 2) K17279 - receptor expression-enhancing protein 5/6 (REEP5_6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTGGCCAACTGCTGTCGACAAGCGTCCC |
| Internal bar code: | CGGCAATGATGATGTGGACATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 325 |
| LEAP-Seq percent confirming: | 99.0099 |
| LEAP-Seq n confirming: | 100 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTGGGTATGTCACAACA |
| Suggested primer 2: | CGACGCGTACTGAGTTGTGT |