| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.254863 |
| Chromosome: | chromosome 3 |
| Location: | 8829405 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGCCATGAAATCCTCAGCTGCAAGAAA |
| Internal bar code: | ATATACCAATCGAGGATCCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 99.505 |
| LEAP-Seq n confirming: | 402 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTTACAGGCGGAATGCAG |
| Suggested primer 2: | ATGGGACATGGAGCTGAATC |