| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.254877 |
| Chromosome: | chromosome 4 |
| Location: | 2428223 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221400 | (1 of 2) IPR014347 - Tautomerase/MIF superfamily | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGAGGTGGCAGCCCCAGAACGTCCTGGG |
| Internal bar code: | AGTTCGTCCTCCATCGGCGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 99.3256 |
| LEAP-Seq n confirming: | 1031 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCATCATGCGGTAACAA |
| Suggested primer 2: | GAGAAGAATGGGAGGAAGGG |