| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.254929 |
| Chromosome: | chromosome 13 |
| Location: | 1737129 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574500 | FER2 | (1 of 2) K00522 - ferritin heavy chain (FTH1); Ferritin subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTATACACAGGCATGTGCACCAGCTTC |
| Internal bar code: | TCTCAGAGTTTATTTCGGCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 206 |
| LEAP-Seq percent confirming: | 99.6755 |
| LEAP-Seq n confirming: | 2457 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTAGTAGTGCGATGCCA |
| Suggested primer 2: | TCGTCCCACCTTTACAGACC |