| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254945 |
| Chromosome: | chromosome 8 |
| Location: | 3815646 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377450 | (1 of 1) PTHR10997:SF9 - IMPORTIN-9 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATTGCTGCTTCGGGCCCCTTTCATCACA |
| Internal bar code: | GCGGGTGCCCTTGTGTTTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 99.7671 |
| LEAP-Seq n confirming: | 2570 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGGGGACTGGGTTAGAG |
| Suggested primer 2: | TTTGCAAGTCGTTGCTTACG |