| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254957 |
| Chromosome: | chromosome 7 |
| Location: | 1204230 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321100 | (1 of 1) PTHR10196:SF67 - SEDOHEPTULOKINASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACTTATCTGACATACCGCCTTTACGCG |
| Internal bar code: | GGGCCTCCGGCTCCCCAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 330 |
| LEAP-Seq percent confirming: | 99.6845 |
| LEAP-Seq n confirming: | 316 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGTGTAACAGCATGGGC |
| Suggested primer 2: | GGACCCTTAGGCCTCATCTC |