| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.254957 |
| Chromosome: | chromosome 7 |
| Location: | 1204323 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321100 | (1 of 1) PTHR10196:SF67 - SEDOHEPTULOKINASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAAGGGGGCTGGGGGAGTCGGGAGGGGCA |
| Internal bar code: | TTCGACTGCCGTAATGAGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 482 |
| LEAP-Seq percent confirming: | 98.2249 |
| LEAP-Seq n confirming: | 166 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGTGTAACAGCATGGGC |
| Suggested primer 2: | CCCTCGAGCCTCTAACTCCT |