| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254989 |
| Chromosome: | chromosome 9 |
| Location: | 2104644 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393750 | (1 of 1) PTHR24031:SF284 - DEAD-BOX HELICASE DBP80 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTGGTCTTGTGTGGCGCATCCCCAGAC |
| Internal bar code: | GATCGTCTCTGCGTCGGCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 116 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 100 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGTGCCAACTTCTAATGT |
| Suggested primer 2: | CCAGGGTTGCTAAAGCTCTG |