| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.255001 |
| Chromosome: | chromosome 1 |
| Location: | 5051165 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034900 | CDPK5 | (1 of 4) PTHR24347:SF138 - PROTEIN F32D8.1; Calcium/calmodulin-dependent protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACTAATGTTCACCACGCGCGTGTCCAAT |
| Internal bar code: | TTACACATGCGGGTCCCTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 29 |
| LEAP-Seq percent confirming: | 99.4901 |
| LEAP-Seq n confirming: | 1756 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGTGGGTTCATTGCTGA |
| Suggested primer 2: | TGGATGGTGGGGTATTAGGA |