| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255004 |
| Chromosome: | chromosome 14 |
| Location: | 1723820 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619500 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACTGGAGCCGGGACTGTCCCCTCTGTAA |
| Internal bar code: | GTTGATTAAGGGCCGTAGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 222 |
| LEAP-Seq percent confirming: | 57.8947 |
| LEAP-Seq n confirming: | 308 |
| LEAP-Seq n nonconfirming: | 224 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTCTGCCCTCTGCTATC |
| Suggested primer 2: | GTATGTGCGATGGGACAGTG |