Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.255039 |
Chromosome: | chromosome 14 |
Location: | 4005472 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g633300 | (1 of 1) IPR003582//IPR006626//IPR009030 - ShKT domain // Parallel beta-helix repeat // Insulin-like growth factor binding protein, N-terminal | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACTACAAATAAGCTCAACAAGCCACAAG |
Internal bar code: | CCGGGTTACGGCCCGACGGGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 456 |
LEAP-Seq percent confirming: | 95.2445 |
LEAP-Seq n confirming: | 7050 |
LEAP-Seq n nonconfirming: | 352 |
LEAP-Seq n unique pos: | 152 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGAGGGGCAAGAAGGAGAAG |
Suggested primer 2: | ACACGTAGACACTGCATCGC |