| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.255047 |
| Chromosome: | chromosome 4 |
| Location: | 2357194 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220850 | (1 of 1) 3.6.3.34//3.6.3.43//3.6.3.44 - Iron-chelate-transporting ATPase // Peptide-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTGCTTGGGCTGGCAATGTTAGGTAAGT |
| Internal bar code: | GTGCTGACAGCGGTCCCCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 99.4937 |
| LEAP-Seq n confirming: | 393 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGGGTGCTGGTTTTGTT |
| Suggested primer 2: | ACTGCCAGTTATGGGTCCTG |