| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.255047 |
| Chromosome: | chromosome 14 |
| Location: | 1292546 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616700 | (1 of 2) 2.6.1.57 - Aromatic-amino-acid transaminase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATCAGACAGAAGGCTCACTTGAGCGCGT |
| Internal bar code: | CAGGTAGCGGAGGCGTACCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 562 |
| LEAP-Seq percent confirming: | 99.6743 |
| LEAP-Seq n confirming: | 306 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGGACTAGCCGAAGCAA |
| Suggested primer 2: | TTGAGGCCTATCTGGTTTGG |