| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255060 |
| Chromosome: | chromosome 6 |
| Location: | 1972941 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263950 | (1 of 1) 3.6.3.9 - Sodium/potassium-exchanging ATPase / Sodium/potassium-transporting ATPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGTTTGCAGTGGCGCCGAGGTCTGGACC |
| Internal bar code: | GGTGGATCAAGAGCATCTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 98.4026 |
| LEAP-Seq n confirming: | 924 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAATCTCCACGCCCCTACA |
| Suggested primer 2: | CACTCCTGACTCCACTGCAA |