| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255142 |
| Chromosome: | chromosome 12 |
| Location: | 8124503 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551350 | LAO2 | (1 of 1) 3.5.99.5 - 2-aminomuconate deaminase; Periplasmic L-amino acid oxidase, accessory subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGAGCTGGTGAGCACCGGGGGGGCGAGG |
| Internal bar code: | TGTCTTCCCCCCGCATTGTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTATGCTTGCAGATCCGAG |
| Suggested primer 2: | CTCTTCTCCTGTCTCACCCG |