| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255168 |
| Chromosome: | chromosome 12 |
| Location: | 7414924 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556700 | (1 of 3) IPR000104//IPR011009 - Antifreeze protein, type I // Protein kinase-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTTGGTCAACCCACCGGAATCGCGCCG |
| Internal bar code: | GGGGAGCTGTAGTTCAAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 414 |
| LEAP-Seq percent confirming: | 94.898 |
| LEAP-Seq n confirming: | 93 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGATCACCACCTCCGTGTA |
| Suggested primer 2: | CTTTGCGAGCCGAGATTTAG |