| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255169 |
| Chromosome: | chromosome 10 |
| Location: | 3859288 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g447550 | (1 of 2) PF03200 - Glycosyl hydrolase family 63 C-terminal domain (Glyco_hydro_63) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAACATGGTGCCCTGCCCCCTGCTGGCC |
| Internal bar code: | GACGACAATGCCACTATCGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 150 |
| LEAP-Seq percent confirming: | 49.7268 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGACTGAATGGCGAATTT |
| Suggested primer 2: | GCTGAGGTAGCCAGGTTGAG |