| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.255177 |
| Chromosome: | chromosome 3 |
| Location: | 8351417 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g198800 | (1 of 1) PTHR24078:SF169 - DNAJ HOMOLOG SUBFAMILY C MEMBER 17 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCGGCAGAGCGGTCGACACGGGATTCT |
| Internal bar code: | GGGTTTAGGAAACGGCAAATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 99.6298 |
| LEAP-Seq n confirming: | 5382 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCATACCTGTTGTGAGGG |
| Suggested primer 2: | CAGACAGGCCCAGAAGTAGC |