| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255196 |
| Chromosome: | chromosome 1 |
| Location: | 5177524 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036050 | MLH1 | DNA mismatch repair protein; (1 of 1) K08734 - DNA mismatch repair protein MLH1 (MLH1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCAACTGCCTCTACCGCACACAGCTCGT |
| Internal bar code: | TGGTGCGCTGGATATCCACCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 429 |
| LEAP-Seq percent confirming: | 99.8282 |
| LEAP-Seq n confirming: | 581 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTAATGGAAGGTGTTGA |
| Suggested primer 2: | GGTTGTGGGCTTGTGAAAGT |