| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255210 |
| Chromosome: | chromosome 1 |
| Location: | 7654516 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g055400 | (1 of 1) PF05022 - SRP40, C-terminal domain (SRP40_C) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGATGGGGCCGGGCGGCTCTGGTCGGGTC |
| Internal bar code: | TGACAGGGCACGCCACCAATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 99.3902 |
| LEAP-Seq n confirming: | 326 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTGCAGCAAAGAAGAAG |
| Suggested primer 2: | GGGCAGTAGCAATAGCTGGA |