| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.255233 |
| Chromosome: | chromosome 12 |
| Location: | 486906 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493300 | (1 of 6) PTHR10582 - TRANSIENT RECEPTOR POTENTIAL ION CHANNEL PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCGCCTCGCTGCGGCCAGGCTGAGCCA |
| Internal bar code: | GAGACCGACCGGCGTGATGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 104 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGAAATTGGCTGCATCTC |
| Suggested primer 2: | ATGATGGAGCTGGACGAAAC |