| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255255 |
| Chromosome: | chromosome 7 |
| Location: | 5228638 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348900 | DNJ19 | (1 of 1) K09537 - DnaJ homolog subfamily C member 17 (DNAJC17); DnaJ-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAAGCCCAAGTCGCAACACCCAAGTCGC |
| Internal bar code: | TCCGCCCTGATTGACGGCTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 387 |
| LEAP-Seq percent confirming: | 54.7046 |
| LEAP-Seq n confirming: | 500 |
| LEAP-Seq n nonconfirming: | 414 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGTTAGCAGGTCTCTCAG |
| Suggested primer 2: | ATTATCCTTGCTACGCCCCT |