| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.255275 |
| Chromosome: | chromosome 10 |
| Location: | 1130125 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425650 | (1 of 4) PF03006 - Haemolysin-III related (HlyIII) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTTGCCATGTCACCGTACGACATGCAA |
| Internal bar code: | GGTTTCTGGGAGCAACTATCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 99.9144 |
| LEAP-Seq n confirming: | 1167 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACACCCATGCTCCAATTC |
| Suggested primer 2: | ATGACCACCTTCTGGTGAGG |