| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.255280 |
| Chromosome: | chromosome 2 |
| Location: | 5049079 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104600 | (1 of 1) PTHR24148:SF18 - ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATAGATCCGGCCGGCGCCTAACAATTGC |
| Internal bar code: | CTCGTGTTGGATACGCATGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 97.3082 |
| LEAP-Seq n confirming: | 723 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTTGCTTCAGAGCAAGGG |
| Suggested primer 2: | ACCGCGTAAAGTAAGCGAGA |