| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.255286 |
| Chromosome: | chromosome 16 |
| Location: | 1565147 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653601 | MRPL33 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTATTGTGTATTGTGTATATAGCAATAA |
| Internal bar code: | GTTGGAGATTCGGAGTACTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 98.9177 |
| LEAP-Seq n confirming: | 457 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCTGGCTAGATACGTCC |
| Suggested primer 2: | TGCCTGAGGTCACTCACTTG |