| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.255300 |
| Chromosome: | chromosome 3 |
| Location: | 2294268 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g158150 | (1 of 1) K12842 - U2-associated protein SR140 (SR140) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCATCAGCTACCGCCGCCGCCGCGTC |
| Internal bar code: | ATCAGATAATAGTTCGGCGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 21.6374 |
| LEAP-Seq n confirming: | 111 |
| LEAP-Seq n nonconfirming: | 402 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTCCCTGACCCGAACTTT |
| Suggested primer 2: | GTCCTTGTCCTTGCTCTTGC |