| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255308 |
| Chromosome: | chromosome 1 |
| Location: | 1957423 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010550 | HLD2 | Haloalkane dehalogenase-like hydrolase; (1 of 2) 3.1.3.76 - Lipid-phosphate phosphatase / Soluble epoxide hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGTGAGGTCACCGGATCGATCCCGGTA |
| Internal bar code: | CTAATGGGGGTTTGCTAAGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 391 |
| LEAP-Seq percent confirming: | 98.8235 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAAAGAGAAGGGGAGGGT |
| Suggested primer 2: | GGTCCTCTCACGTCACCAAT |