Insertion junction: LMJ.RY0402.255321_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.255321
Chromosome:chromosome 13
Location:2777502
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGCCCCGTGCTGCCCCATGCTCACACG
Internal bar code:TTGAGGTCAGTCGGAACCCCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:135
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCACCCCTCCTTATCAAC
Suggested primer 2:CTTACTTCGCTTCGCTTGCT