Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.255326 |
Chromosome: | chromosome 6 |
Location: | 5451968 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g284376 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACATCTACCTCATCGTTCAAACATAGCC |
Internal bar code: | GTTTCACGAAGGTGCGCACTAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 611 |
LEAP-Seq percent confirming: | 98.6348 |
LEAP-Seq n confirming: | 867 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTTGGACTGCTTTGTTGCTG |
Suggested primer 2: | CACACACACACACACACGGT |