Insertion junction: LMJ.RY0402.255326_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.255326
Chromosome:chromosome 6
Location:5451968
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g284376 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACATCTACCTCATCGTTCAAACATAGCC
Internal bar code:GTTTCACGAAGGTGCGCACTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:611
LEAP-Seq percent confirming:98.6348
LEAP-Seq n confirming:867
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGGACTGCTTTGTTGCTG
Suggested primer 2:CACACACACACACACACGGT