| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255336 |
| Chromosome: | chromosome 10 |
| Location: | 2348030 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g434967 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTAATTCCAGCTATTTATTTACCCCCC |
| Internal bar code: | AGGGCTCCTCAGGTCGGGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 177 |
| LEAP-Seq percent confirming: | 97.2973 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAAGTGGAGGAGGAGGAG |
| Suggested primer 2: | CCCTAAACGCCCCTCTAAAC |