| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.255339 |
| Chromosome: | chromosome 17 |
| Location: | 5185632 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g735600 | TGL21 | (1 of 3) PTHR21493//PTHR21493:SF153 - CGI-141-RELATED/LIPASE CONTAINING PROTEIN // PROTEIN T08B1.4, ISOFORM B-RELATED; Putative triacylglycerol lipase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGCGCAGCTATCGAGTTGAGCACTCGA |
| Internal bar code: | CTGGTTATCTCGGCGAGTCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 569 |
| LEAP-Seq percent confirming: | 99.4269 |
| LEAP-Seq n confirming: | 347 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTCTTCTCGTGTCTGGC |
| Suggested primer 2: | AGCGTGCAGGTAGTCATGTG |