| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.255354 |
| Chromosome: | chromosome 10 |
| Location: | 3338768 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443801 | (1 of 3) PF13401 - AAA domain (AAA_22) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTGCGTTGGGTCCCGCGAGGCCTCGTGA |
| Internal bar code: | ATCGAGGGCAGGGGCACATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1068 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCATCCTGTACCGCTTTT |
| Suggested primer 2: | ATGATTGCTTCACTTTGCCC |