Insertion junction: LMJ.RY0402.255359_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.255359
Chromosome:chromosome 5
Location:667222
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g230900 DPP1,PAP1 (1 of 3) PTHR10165:SF35 - DIACYLGLYCEROL PYROPHOSPHATE PHOSPHATASE 1-RELATED; Diacylglycerol pyrophosphate phosphatase 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACCAATACCGGGCGAAGATCGTCTTACC
Internal bar code:CGGCACTCGGTCTAATACGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:472
LEAP-Seq percent confirming:98.3462
LEAP-Seq n confirming:892
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AGCGCTCCAACTACGAGGTA
Suggested primer 2:TAGTTGGCAGTTGGCAGTTG