Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.255377 |
Chromosome: | chromosome 13 |
Location: | 3411568 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g587200 | PIGW1,PIGW | Inositol acyltransferase, PIG-W; (1 of 1) K05283 - phosphatidylinositol glycan, class W (PIGW) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGTGGTTGCGGTGGCGGTGGTAGCGG |
Internal bar code: | GGCGTTTGTCATACCGGAAAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 93 |
LEAP-Seq percent confirming: | 96.4459 |
LEAP-Seq n confirming: | 597 |
LEAP-Seq n nonconfirming: | 22 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTTCAACCACACAGCATGG |
Suggested primer 2: | CGTACATCAGCACACGCTTT |