Insertion junction: LMJ.RY0402.255380_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.255380
Chromosome:chromosome 17
Location:4627188
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g733150 HKR7,HKR78,HKR8,COP12HKR6,COP,COP11 Histidine kinase rhodopsin 7/8; (1 of 1) PF00072//PF00512//PF01036//PF02518 - Response regulator receiver domain (Response_reg) // His Kinase A (phospho-acceptor) domain (HisKA) // Bacteriorhodopsin-like protein (Bac_rhodopsin) // Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCACCTTGCCGGTCGCCCGGGTGTGTTG
Internal bar code:GCCTGTAACGACCGACAGTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:617
LEAP-Seq percent confirming:99.5516
LEAP-Seq n confirming:444
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTAGAGGCGTGGTTTGTA
Suggested primer 2:ACAGATTGCGCAGCCTAGAT