Insertion junction: LMJ.RY0402.255391_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.255391
Chromosome:chromosome 16
Location:1301982
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g651500 (1 of 1) K12180 - COP9 signalosome complex subunit 7 (COPS7, CSN7) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTAGTGGCGGTCGCGGGGGAGTCGGTT
Internal bar code:ATCGTTCCCGGCGTTCTGCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:490
LEAP-Seq percent confirming:99.2711
LEAP-Seq n confirming:681
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGTCAGTACGGTCTGTGG
Suggested primer 2:CGGCTGCTAGCTCTCAAGTT