Insertion junction: LMJ.RY0402.255397_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.255397
Chromosome:chromosome 7
Location:5321923
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g349750 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCAGCACACACACCGCCCAGCAAACCCA
Internal bar code:TTTGTCGGAATTTTATATTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:84.2143
LEAP-Seq n confirming:2342
LEAP-Seq n nonconfirming:439
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGACGTGCCAGTATCAGA
Suggested primer 2:GGACTGACGGGCACTGTAAT