| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.255416 |
| Chromosome: | chromosome 3 |
| Location: | 5567005 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186550 | (1 of 1) K11137 - telomere length regulation protein (TELO2, TEL2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAACGCACCTGCATGCAGTCGTACGGCAG |
| Internal bar code: | CTTCGGAGCGTAATCTCCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 86 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 280 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTGTAGCATACGAGCTG |
| Suggested primer 2: | ATTGTACTTCAACGCTGGGC |