| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255419 |
| Chromosome: | chromosome 12 |
| Location: | 3126447 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499750 | (1 of 89) PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) (RRM_1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTTCTGATGCGCTTGGCCCACGCGGGT |
| Internal bar code: | ACGAGGATGCATTTCTTGGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 401 |
| LEAP-Seq percent confirming: | 87.9518 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCCTTGAGAGAGGGAGT |
| Suggested primer 2: | TACCTACGGCTTCGTCTCGT |